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CRISPR / Cas9系統(tǒng)是用于基因編輯的RNA引導(dǎo)核酸內(nèi)切酶技術(shù)。該系統(tǒng)需要包含與PAM（Protospacer Adjacent Motif）位點(diǎn)相鄰的20bp靶序列的指導(dǎo)RNA（gRNA），以將Cas9核酸內(nèi)切酶引導(dǎo)至切割位點(diǎn)。Geneious中的Find CRISPR網(wǎng)站工具將搜索所選基因中的gRNA（“CRISPR”）位點(diǎn)，并在您感興趣的基因組中搜索脫靶結(jié)合位點(diǎn)。選定序列中的每個CRISPR站點(diǎn)根據(jù)它可能會綁定到多少個脫離目標(biāo)站點(diǎn)以及脫離目標(biāo)站點(diǎn)與原始序列的相似程度進(jìn)行評分。
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